Total flavonoid content and in vitro study on the sunscreen activity of extracts of leaves of Elaeocarpus floribundus blume

Background: Elaeocarpus floribundus blume leaves have traditionally been used to treat a variety of diseases; Its hot water infusion is as gargle inflamed gums and relieve rheumatoid arthritis. Furthermore, previous research has shown that its methanol extract contains high level total phenolic content. Objective: To determine the flavonoid content (TFC) E.floribundus extracts conduct preliminary on their sunscreen activity. Method: This plant's dried-bulk were macerated in three different solvents, n-hexane, ethylacetate, methanol, order. The TFC all was determined using colorimetric method with AlCl3 reagent quercetine standard. In contrast, activity assessed by determining value Sun Protection Factor (SPF) UV-Vis spectrophotometer 3-benzophenone positive control. Result: results indicated ethyl acetate, contained 35.58 ± 1.87 mgQE/g extract, 60.59 0.53 149.28 0.89 respectively. With SPF values 45.470; 44.791; 43.754; 38.861 21.102 for tested concentrations 1000; 800; 600; 400 200 mg/mL, respectively, demonstrated highest potential sun protection However, this not more potent than 3-benzophenone, which had an 22,647 at concentration 50 mg/mL. Conclusion: E. blume's greatest among extracts.